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|Title: ||Pollen Conservation as Nuclear Genetic Diversity for improvement of Citrus|
|Authors: ||S, Ganeshan|
|Issue Date: ||2010|
|Citation: ||Ganeshan S. Pollen Conservation as Nuclear Genetic Diversity for improvement of Citrus. National Seminar on Citrus Biodiversity for Livelihood and Nutritional Security. 4 – 5 October, NRCC, Nagpur|
|Abstract: ||Citrus Pollen preservation aids and supplements conservation efforts that traditionally involve seed or vegetative plant maintenance. The importance of citrus breeding for releasing new cultivars to citrus industry is well known. Research to explore the best method of pollen preservation in citrus for use in breeding was globally initiated to optimize the badly required protocols. Efforts to accomplish long-term pollen storage in citrus were made with an objective to improve breeding efficiency and solving issues related to asynchrony in flowering among crossing parents. At IIHR Bangalore, research on citrus pollen storage was initiated in the late 70s and early 80s in the Division of Plant Genetic Resources. Storage of pollen at low temperature was successful with Citrus limon pollen for short term durations, but a reduction of germination profile was observed with increasing storage period. With further reduction of temperature to sub-zero levels up to -18OC, pollen remained viable up to a period of 6 months without losing viability. Cryogenic storage was successfully accomplished with citrus pollen. Inter-generic crosses attempted with cryopreserved Poncirus trifoliata pollen over Citrus limonia resulted in the generation of zygotic seedlings expressing the trifoliate leaf character in the resulting F1 progeny. The presentation will review the research status of pollen conservation in citrus and allied genera for breeding needs and importance of establishing cryo-repositories of the haploid genome for breeding needs and conserving nuclear genetic diversity.|
|Appears in Collections:||Plant Genetic Resources|
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