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|Title: ||Colchicine induced variability: Impact on pollen grains and stomata of interspecific hybrid in Dianthus.|
|Issue Date: ||2007|
|Citation: ||Tejaswini, Neeraj Tripathi and Paramesh H. 2007. Colchicine induced variability: Impact on pollen grains and stomata of interspecific hybrid in Dianthus.Plant Breeding News. 176: 1.19|
|Abstract: ||In an effort to restore reproductive ability and to improve ornamental characteristics, in vitro colchicine treatment was attempted in a selected interspecific hybrid of Dianthus. Protocols of soaking explant in colchicine prior to tissue culture as well as culturing the explant on media incorporated with the colchicine were tried to optimize the oncentration for creating maximum variability and to enhance pollen viability. Explants were soaked in various concentration of colchicine in combination with varying treatment duration. The resulting in vitro plantlets were hardened and were observed for pollen and stomatal characters. Attempt was made for rapid and early detection of variability by screening pollen and stomata size. Pollen grain size and percentage of viable pollen grains increased with increased colchicine concentration and treatment duration. Concentration of colchicine significantly influenced the pollen viability as well as size of viable pollen grains. Significant impact of colchicine concentration was also noticed for stomatal number and size on both upper and lower surface of leaf.|
|Appears in Collections:||Ornamental Crops|
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