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|Title: ||The origin and development of secondary somatic embryos in banana cv. Nanjangud rasbale (silk gp. Rasthali, AAB).|
|Authors: ||Sukhada Mohandas|
K Y, Prathibha
H D, Soumya
R M, Ranganath
|Issue Date: ||2010|
|Citation: ||Sukhada, M., Prathibha, K.Y., Soumya, H.D. and Ranganath, R.M. 2010. The origin and development of secondary somatic embryos in banana cv. Nanjangud rasbale (silk gp. Rasthali, AAB). Acta Hort. (ISHS) 865:349-35.|
|Abstract: ||Studies were initiated to develop regeneration protocol through somatic embryogenesis in banana cv. Rasthali. Embryogenic callus and somatic embryos were generated with male flower buds on MS medium supplemented with 2,4-D (18.10 μM ), NAA (5.37 μM) and IAA (5.71 μM ) with 3% sucrose and 0.2% gelrite. After 6–8 months well developed somatic embryos were sub cultured on to MS supplemented with NAA (1.07 µM), Zeatin (0.23 µM) ,2-ip (0.60 µM) and kinetin (0. 46 µM) for further maturation. Plantlets were obtained when somatic embryos were cultured on MS with Morel vitamin, IAA (11.42 µM)), and BAP (2.22 µM). Formation secondary somatic embryos on the primary embryos were observed if the primary embryos were left in the induction media a little longer (8–9 months). Transparent, fragile, secondary buds emerged either on top of young globular embryos or on cotyledonary region of matured primary somatic embryos. Histological and histochemical studies were carried out to verify the mode of development of secondary somatic embryos on the primary somatic embryos and it revealed that the secondary embryos were formed directly on the epidermis of primary somatic embryos without intervening callus. The study has shown two modes of development of direct secondary embryogenesis. In the first mode, the secondary somatic embryos originated from single epidermal cells of the young globular primary embryos. In the second mode several epidermal cells of matured primary somatic embryo were involved in the formation of secondary somatic embryos. Localization of starch, total insoluble proteins and nucleic acids were seen in the secondary somatic embryos.|
|Appears in Collections:||Biotechnology|
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