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|Title: ||Assessment of genetic relatedness among mango cultivars of India using RAPD markers|
|Authors: ||Ravishankar, K V|
Dinesh, M R
|Issue Date: ||2000|
|Publisher: ||Journal of Horticultural Science and Biotechnology|
|Citation: ||Assessment of genetic relatedness among mango cultivars of India using RAPD markers, Journal of Horticultural Science & Biotechnology ,75 (2) 198-201|
|Abstract: ||DNA – based RAPD (Random, Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in a number of fruit crops. A wide genetic diversity exists in the mango fruit in India. Present day commercial cultivars originated mainly from this subcontinent. In this study, 18 commercial mango cultivars, traditionally grown in western, southern, northern and eastern parts of India were selected to assess genetic relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 30 arbitrary 10 - mer primers, of these 27 primers amplified mango genomic DNA. None of these primers produced unique band pattern for each cultivar. RAPD data were used to calculate a squared Euclidean distance matrix, and based on this cluster analysis was done using a minimum variance algorithm. Cluster analysis clearly showed two group – the first consisting of western. Northern and eastern mango cultivars and the second groups- the first consisting of western Northern and eastern mango cultivars and the second group consisting of southern cultivars. From the analysis of results, it appears the majority of mango cultivars originated from a local mango genepool and were domesticated later.|
|Appears in Collections:||Fruit Crops|
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