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Please use this identifier to cite or link to this item: http://www.erepo.iihr.ernet.in/handle/123456789/268

Title: Ubiquitous presence of fastidious endophytic bacteria in filed shoot and index- negative apparently clean shoot- tip cultures of papaya’
Authors: Pious, Thomas
Sima, Kumari
Ganiga, K
Devalakere P Prakash Swarna
M R, Dinesh
Keywords: Carica papaya
micro-propagation
Endophytic bacteria
Indexing
Issue Date: 2007
Publisher: Plant Cell Reporter
Citation: Ubiquitous presence of fastidious endophytic bacteria in filed shoot and index- negative apparently clean shoot- tip cultures of papaya’. Plant Cell Rep 26:146-1499
Abstract: This study establishes the widespread prevalence of fastidious or viable but non- culturable endophytic bacteria in unsuspicious shoot –tip cultures of papaya (Carica papaya L.) against the norm of asepsis in vitro. A total of 150 shoot – tips (approximately 10 mm) were inoculated on Ms-based culture medium after surface sterilization of field-derived auxiliary shoots of cv. Surya during November or January(100 and 50, respectively) when 35-50% cultures showed endophytic microbial growth on culture medium. Indexing of apparently clean cultures using bacteriological media helped in detecting and removing additional 14 -17 % stocks with covert bacteria during the first two passages. The rest of the stocks stayed consistently index-negative during the first subculture cycles, but appeared positive in PCR product yielded overlapping nucleotide data signifying mixed template or the presence of diverse endophytic microorganisms. This was confirmed by light microscopy of tissue sap revealing viable bacteria in considerable number, which were detected under phase contrast or with negative staining. Planting tissue segments or applying homogenate from these stocks on diverse bacteriological media did not induce the organisms to grow in vitro. The shoot cultures displayed variation in growth and rooting potential, the onus of such variation was solely attributable to the associated microorganisms. The findings were confirmed with additional filed shoots and fresh in vitro stocks established subsequently. The observations have implications in micro propagation and all other applications involving plant cell, tissue, organ, and protoplast culture.
URI: http://hdl.handle.net/123456789/268
Appears in Collections:Fruit Crops

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