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Title: Validation of molecular markers linked to ToLCV resistance in tomato varieties/F1 hybrids
Authors: Shamprasad Phanis
Guide/Chairperson: A T, Sadashiva
Keywords: molecular markers
F1 hybrids
Issue Date: 2010
Year of Submission: 2010
Abstract: Tomato (Solanum lycopersicum L.) is one of the most important and extensively grown vegetables around the world. Successful cultivation of tomato crop has been hindered due to attack by numerous pests and devastating diseases. Chiefly of these limiting factors, the tomato leaf curl disease caused by Tomato Leaf curl Virus (ToLCV) is a destructive disease of tomato in many parts of India and world. The use of molecular markers linked to genes for resistance is a tool, which can be used efficiently in plant breeding through marker assisted selection (MAS). In this study three molecular markers Ty1, Ty2 and Ty3 linked to ToLCV resistance were validated with fourteen ToLCV resistant entries after screening against ToLCV resistance under screen house. These resistant entries included four ToLCV resistant varieties (Hisar Anmol, Vyabhav, Nandhi and Sankranthi), six ToLCV resistant commercial hybrids (Abhinava, Arka Ananya, Lakshmi, NS-501, Shakthiman and US-618); three advanced breeding lines (IIHR-2611, IIHR-2822 and IIHR-2823) and a ToLCV resistant wild accession Solanum habrochaites LA 1777 (IIHR-2101) and Pusa Ruby was used as a susceptible check. Among these, two advanced breeding lines IIHR-2822 and IIHR-2823 showed the presence of the all three genes TY1, Ty2 and Ty3 for ToLCV resistance, the wild accession S. habrochaites LA 1777 (IIHR- 2101) showed the presence of two genes Ty2 and Ty3, Abhinava showed the presence of Ty1 gene and Hisar Anmol (H-24), Vyabhav, Arka Ananya, Lakshmi, NS-501 showed the presence of only Ty2 gene and none of them showed the Ty3a gene presence. The varieties Nandhi and Sankranthi; hybrids Shakthiman and US-618 and the advanced breeding line IIHR-2611 (TV 55) did not show any presence of the Ty1, Ty2 and Ty3 resistant genes.
URI: http://www.erepo.iihr.ernet.in/handle/123456789/373
University in which they received their degree: University of Agricultural Sciences, Bangalore
Degree Level: M.Sc

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