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Title: Assessment of genetic variability and identification of DNA markers linked to fruit quality traits in chilli (Capsicum annuum L.)
Authors: Ponnam Naresh
Guide/Chairperson: K, Madhavi Reddy
Keywords: genetic
DNA markers
Issue Date: 2011
Year of Submission: 2011
Abstract: Chilli is an indispensable commodity in every Indian cuisine due to its pungency, spicy taste, appealing colour and flavour of fruits. Understanding genetic variability and population structure is of great importance and a prerequisite for association mapping to identify marker trait associations. Investigation on Genetic variance for major fruit quality traits was carried using 116 chill genotypes. The high GCV and PCV were observed for total capsaicinoids, total carotenoids, red and yellow fractions of total carotenoids and total soluable solids. High heritability coupled with high GAM was observed for all the major quality traits studied. Investigation on population structure was carried out with 100 chilli genotypes using 24 genome-wide simple sequence repeat (SSR) markers using STRUCTURE and DARwin softwares. The 100 accessions were divided into three subpopulations. The three clusters seemed to have very distinct parentages with few genotypes categorized as having admixed ancestry from at least two clusters and represented a good population structure which is amenable for association analysis. Allele mining approach was followed to see Sequence variation in candidate genes responsible for colour and pungency. Results revealed that Capsanthin capsorubin synthase gene coding region was present in yellow pepper line LCA 1068 (Aparna) with 99% similarity to sequence of Byadagi Dabbi (dark red line) except for a single nucleotide polymorphism (G935A) and an amino acid change (R312K). In case of pungency, Acyltransferase gene several single nucleotide polymorphisms (SNPs) were found scattered throughout the amplified sequence of 840bp. Phylogenetic analysis of sequences showed clear demarcation between AT3-1 and AT3-2 (tandem duplicates of AT3) and the non-pungent lines are not clustered into single cluster suggesting the existence of contrasting modes for varied level of pungency.
URI: http://www.erepo.iihr.ernet.in/handle/123456789/397
University in which they received their degree: University of Agricultural Sciences, Bangalore
Degree Level: M.Sc

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