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Title: Induction of mutation in carnation (Dianthus caryophyllus L.) through gamma rays and ethyl methane sulphonate.
Authors: K, Hemalatha
Guide/Chairperson: R N, Bhat
Keywords: Induction
gamma rays
ethyl methane sulphonate
Issue Date: 1998
Year of Submission: 1998
Abstract: Induction of mutation was attempted on two carnation genotypes viz., ‘Sterlite Dop’ and ‘H-13’ using gamma rays as physical mutagen and on one genotype ‘Sterlite Dop’ using EMS as chemical mutagen at different concentrations. The effect of mutagen treatments on various morphological and floral characters, histological and histochemical changes during rhizogenesis and organogenesis and pigment composition were studied. Increased dosage decreased sprouting and survival of cuttings. Induction of mutation reduced all the morphological characters at higher dosage of gamma rays. Some interesting leaf variegations like leaves with white and creamy yellow streaks were observed in cuttings treated at 1.5 and 2 kR gamma rays. Interesting changes were observed with respect to the floral characters. At 2 kR gamma rays, petal variegation was observed in genotype ‘H- 13’. Two flower colour mutants were observed in cutting treated at 1.5 kR gamma rays in genotype ‘Sterlite Dop’ and 1 and 2 kR gamma rays in genotype ‘H-13’. A miniature mutant with reduction in flower size was also observed in genotype ‘Sterlite Dop’ treated at 2 kR gamma rays. Induction of mutation through EMS showed very little response. However, few plants treated with 3% EMS showed creamy yellow streaks on the leaves of cv. Sterlite dop. Correlation studies revealed that environmental and genetic factors influenced to a great extent. Meager deviation in genotype and phenotopic co-efficient indicated the reliability of variation. Histological and histochemical studies of the shoot apex revealed the stepwise development of cellular damages like suppression in the growth of dome shaped apex, vacuolation of cells, damage of the apical initial layer, total damage of a small percentage of cells and formation of dichotomy with the extent of damage increasing in the dosage of gamma rays. Exposure of cutting to different doses of gamma rays resulted in correlative inhibition of root emergence. Considerable variation was observed in the anthocyanin, chlorophyll and carotenoid content among the seven carnation genotypes studied. Quantitative and qualitative studies using paper chromatographic and thin layer chromatographic methods were undertaken. By comparing athocyanin bands with that of authentic standards, the pigments present in carnation were tentatively identified as cyanin and pelargonidin.
URI: http://www.erepo.iihr.ernet.in/handle/123456789/408
University in which they received their degree: University of Agricultural Sciences, Bangalore
Degree Level: Ph.D

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