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Please use this identifier to cite or link to this item: http://www.erepo.iihr.ernet.in/handle/123456789/417

Title: Encapsulation & regeneration of protocorms in orchid (Spathoglottis plicate)
Authors: B V, Champa
Guide/Chairperson: Foja Singh
Keywords: Encapsulation
Issue Date: 1995
Year of Submission: 1995
Publisher: Foja Singh
Abstract: Encapsulation is a technique, where in cultured matter like a tissue piece, or an organ, or a cell is coated with synthetic polymer which later degrades and allows the plant to establish. An investigation on Encapsulation & regeneration of protocorms from encapsulated breads das carried out in the orchid laboratory, IIHR, Hessaraghatta, Bangalore during 1993-95. Orchid Seeds from matured pods of spathoglottis plicate were sown on modified vaccine & went medium, Murashige & Skoog medium & Nitsch medium by green pod culture technique to standardise the media for protocorm production. It was found that modified vaccine & went medium; Murashige & Skoog medium gave satisfactory results & resulted in healthy protocorms. The protocorms were encapsulated using different hydrogels viz., sodium alginate, alsinic acid, agar & gelrite. Among these, sodium alginate was found to be best hydrogel for encapsulation. The encapsulated beads were stored in sterile pertriplates, wrapped with parafilm & kept under storage temperatures for long term storage temperature. Beads storage at this temperature, were viable even after 12th week of storage & gave good regeneration when cultured on artificial medium. The regenerated plants, when transplanted to community pots established well with 70-80% field establishment. This technique offers a unique way for efficient delivery system, storage & efficient transport of plant material from one to another place under limited refrigeration conditions. 122
URI: http://www.erepo.iihr.ernet.in/handle/123456789/417
University in which they received their degree: University of Agricultural Sciences, Bangalore
Degree Level: Ph.D

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