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Please use this identifier to cite or link to this item: http://www.erepo.iihr.ernet.in/handle/123456789/488

Title: Development of integrated disease management package and transgenic technology for the control of powdery mildew (Leveillula taurica) in capsicum (Capsicum annuum L.)
Authors: A, Manoj Kumar
Guide/Chairperson: Girija Ganeshan
Keywords: integrated
disease management
transgenic
powdery mildew
capsicum
Issue Date: 2007
Year of Submission: 2007
Abstract: Bell pepper (Capsicum annuum L.) is an important vegetable crop in India and World. Powdery mildew (Leveillula taurica) takes heavy toll under field and greenhouse conditions. The major goal of the study was to develop an Integrated Disease Management (IDM) package for the control of powdery mildew (Leveilula taurica) in bell pepper and development of transgenic bell pepper using Agrobacterium tumefaciens mediated in plant transformation protocol. Five commercial varieties were evaluated for the disease incidence and yield during kharif 2004 and rabi 2005 with five sets of sprays of dinocap @ l ml/lt was found significantly effective in controlling powdery mildew disease and increase in yield of all genotypes. Among the OP varieties, Arka Mohini recorded maximum yield and minimum disease incidence and F1 hybrid, Indra also performed well reporting high fruit yield and lowest disease incidence. Later all these five commercial genotypes were screened using RAPD primers. From 58 primers about 219 unambiguous, readable and reproducible bands were produced, 57 (26%) were polymorphic and shared among at least two individuals, 144 (65.8%) were monomorphic common to all the individuals and 18 (8.2%) were polymorphic and unique. The grouping of varieties with similar morphological features was done based on cluster analysis. The variety grouped under cluster A were high yielding with low disease incidence, indicating that they are hybrids. The varieties grouped under cluster B recorded relatively low yield and high disease incidence. Among thirteen fungicides, eight biological control agents and thirteen plant products evaluated in vitro for the control L. taurica indicated that three fungicides, Triademifon, Tebuconazole and Dinocap, all biological control agents and two plant products viz., neem oil and pongamia oil effectively reduced the powdery mildew spore germination. Fungicides viz., Triademifon, Tebuconazole and Dinocap; biological control agent, Ampelomyces quisqualis and plant products neem oil and pongamia oil significantly reduced the powdery mildew disease incidence and increased the fruit yield for both the seasons in both the varieties. Higher levels of chlorophyll a, b, total chlorophyll, total soluble sugar, total phenols and ortho dihydroxy phenols were recorded in T. viride and T. harizianum treated leaves as against untreated control and other treatments. The increased levels of biochemical constituents induced by T.viride and T.harizianum may play a vital role in plant defense mechanism. Adopting the IDM strategy developed including A.quisqualis, T.harzianum, pongamia oil and best fungicide there was a decline in the powdery mildew disease incidence around 93.0 per cent over control in Indra and California Wonder. In the yield, 122.3 and 342.8 per cent increase over control in Indra and California Wonder respectively. Transgenic technology was standardized to incorporate disease resistance gene. In Arka Gaurav, 45 To transformants were raised and fruits were harvested. 110 T1 plants were raised and screened using gene specific uidA (gus) (463 bp) primers by PCR amplification. 21 PCR positives were identified from T1 generation was identified with an expected fragment size. In T2 generation, 76 plants were analysed for the presence of transgene in which 68 plants were PCR positives against uidA (gus), hpt II and 35s-uidA specific primers. The total RNA was extracted form the selected plants (TAG-2, TAG-6 and T AG-8 of T1 generation) and was reverse transcribed to single stranded cDNA and the ss cDNA was employed as template in a PCR reaction using uidA (gus) specific primers. The transcripts of expected size were present in all the selected plants. Over all the efficiency of transformation was 6.66 per cent. In case of Arka Mohini, 35 To transformants were raised and fruits were harvested. 31 T1 plants were screened using gene specific uidA (gus) (463 bp) primers by PCR amplification. 8 PCR positives were identified from T1 generation was identified with an expected fragment size. Further, these plants were also screened using hpt II (509 bp) and 35suidA (687 bp) specific primers. In T2 generation, 30 plants were analysed for the presence of trans gene in which 14 plants were PCR positives against uidA (gus), hpt II and 35s-uidA specific primers. The total RNA was extracted he selected plants (TAM- 2, TAM- 4 of T1 generation) and was reverse transcribed to single stranded cDNA and the ss cDNA was employed as template in a PCR reaction using uidA (gus) specific primers. The transcripts of expected size were present in all the selected plants. Over all the efficiency of transformation was 5.71 per cent.
URI: http://www.erepo.iihr.ernet.in/handle/123456789/488
University in which they received their degree: Kuvempu University, Shimoga
Degree Level: Ph.D
Appears in Collections:DIVISION OF PLANT PATHOLOGY

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