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Title: Morpho-cultural characterization of indigenous Pleurotus isolates
Authors: A, Yasotha
Guide/Chairperson: Meera Pandey
Keywords: Morpho-cultural
Pleurotus isolates
Issue Date: 2008
Year of Submission: 2008
Abstract: Pleurotus species is a promising mushroom form a group of edible species. Mushroom varieties are at present hard to protect since they are mainly characterized by variable fruit body and yield characteristics. It seems to be morphologically different and is taxonomically discrete. They are greatly influenced by environmental factors. In the present study, six isolates from different geographical regions, Bangalore, Shimoga, Madurai, Udaipur and Banswara (Rajasthan) were studied. All the six isolates showed high degree of variability in terms of colony morphology, media, temperature, mycelial characters and spore production. Pleurotus djamor grew faster than the other Pleurotus species at 25 ºC. Linear growth of mycelium was observed in all the region isolates. The two isolates, P. cystidiosus showed a different kind of growth which produced black heads of coremia on MEA, SSM and RC. Pinkish pigment in the mycelia of P. djamor was produced on MEA, PCA and SSM, which is a characteristic observation. Influence of temperature on the growth of isolates showed variability based on geographical regions. Bangalore isolates showed an optimum of 25 ºC, Shimoga, Madurai and Udaipur isolates of 30 ºC and Banswara (Rajasthan) isolate showed optima of 40 ºC suggesting the existence of variability. Out of six isolates, Bangalore and Udaipur showed a high degree of variability in mycelial thickness in both skeletal and generative hyphal system. There exists variability in the kind of hyphal system also. Bangalore, Shimoga and Udaipur isolates showed Dimitic hyphae, whereas Madurai showed monomitic hyphae. But Banaswara isolate showed trimitic hyphae, which is only showed the existence of variability in all study. Basidiospore and asexual spore characters are considered an important character for species identification. They are similar in structure but size varied. Only P. cystidiosus produced the asexual spore, which is used to distinguish from the other Pleurotus sp. Morphological studies are also used to determine the species. In this, only P. cystidiosus and P. djamor showed a different morphology that is P. cystidiosus showed very large fruiting bodies exists in a single and blackish brown in colour, whereas P. djamor showed pink colour fruiting bodies.
URI: http://www.erepo.iihr.ernet.in/handle/123456789/495
University in which they received their degree: Bharathiar University, Coimbatore
Degree Level: M.Sc

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