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| Title: | A new method for estimation of L-DOPA using HPLC. |
| Authors: | T N, Shivananda
G U, Harish
Vibha, Rao
S, Khanam |
| Keywords: | Mucuna
L-DOPA |
| Issue Date: | 2003 |
| Citation: | T.N. Shivananda, G.U. Harish, Vibha Rao and Salma Khanam. 2003. A new method for estimation of L-DOPA using HPLC. National Academy of Sciences, 26(1 & 2), |
| Abstract: | A new method has been developed for the estimation of L-dopa using HPLC. The new method was compared with already established method for accuracy and reproducibility. In this method L-dopa from Mucuna pruriens seed was extracted using 0.1M orthophosphoric acid. The extracted L-dopa was analyzed in HPLC using sodium orthophosphate buffer (pH 2.8) as mobile phase. The analysis could be done using single pump. The L-dopa peak appeared at retention time 3.5 to 3.8 minutes. The cost per sample was worked out to be Rs. 0.25. |
| URI: | http://hdl.handle.net/123456789/84 |
| Appears in Collections: | Medicinal Crops
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