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|Title: ||A new method for estimation of L-DOPA using HPLC.|
|Authors: ||T N, Shivananda|
G U, Harish
|Issue Date: ||2003|
|Citation: ||T.N. Shivananda, G.U. Harish, Vibha Rao and Salma Khanam. 2003. A new method for estimation of L-DOPA using HPLC. National Academy of Sciences, 26(1 & 2),|
|Abstract: ||A new method has been developed for the estimation of L-dopa using HPLC. The new method was compared with already established method for accuracy and reproducibility. In this method L-dopa from Mucuna pruriens seed was extracted using 0.1M orthophosphoric acid. The extracted L-dopa was analyzed in HPLC using sodium orthophosphate buffer (pH 2.8) as mobile phase. The analysis could be done using single pump. The L-dopa peak appeared at retention time 3.5 to 3.8 minutes. The cost per sample was worked out to be Rs. 0.25.|
|Appears in Collections:||Medicinal Crops|
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