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|Title: ||A putative molecular marker for resistance to Late Blight in Ashwagandha|
|Authors: ||D H, Sukanya|
|Issue Date: ||2010|
|Citation: ||D.H.Sukanya, N. Ramachandran, Gayatri Datta, K.Himabindu. 2010. A putative molecular marker for resistance to Late Blight in Ashwagandha.National Symposium on Molecular Approaches for management of fungal diseases of crop plants.Dec 27-30.IIHR, Bangalore.|
|Abstract: ||Ashwagandha (Withania somnifera Dunal) is the most important commercial medicinal crop of India considered as an alternative to ginseng. Medicinal value is attributed to presence of alkaloids and the withanolides and drug is used as stress reliever, immuno-modulator, memory booster and for treating arthritis, cancer etc. Late blight caused by Phytophthora infestans is the most serious disease on the members of Solanaceae family which may result in complete loss in susceptible germplasm (Ojiambo et al., 2000). Ashwgandha is a host for P. infestans but epidemic damage due to pathogen has not been reported till now. Severe incidence of the disease was noticed on this crop during 2009 at Indian institute of Horticultural Research. Since the crop is under extensive commercial cultivation, studies on locating resistance sources and associated markers is of paramount importance. A total of 186 germplasm were scored for the disease reaction. The symptoms were observed on leaves, stem and fruit and caused mortality in some of the accessions. Reaction ranged from free from any symptoms to complete mortality. Sixteen germplasm comprising resistant and susceptible accessions encompassing both annuals and perennials were considered for the molecular investigation. Based on the report in potato, OPG 2 and OPP 9 RAPD primers were used for amplification. Two polymorphic bands of 550bp and 650bp size were amplified with OPG 2 and only 550bp bands were present in all the resistant accessions while both 550bp & 650bp bands were present in the susceptible ones. Thus the presence of 650 bp band could be used as a marker for Phytophthora infestans susceptibility. The results need further confirmation under artificial inoculation using large set of accessions and conversion of the RAPD into a SCAR marker.|
|Appears in Collections:||Medicinal Crops|
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